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Alternatively, the stock society organisms can be grown in an acceptable liquid medium (i.e., SoybeanCasein Digest Broth or Sabouraud Dextrose Broth) and the cells harvested by centrifugation, then washed and resuspended in sterile saline TS to get a microbial count of about 1 × 108 cfu for each mL. [NOTEThe estimate of inoculum focus